Complex formation influence on reaction rate II. Hydrolytic behavior of riboflavin in borate buffer

✍ Scribed by Deodatt A. Wadke; David E. Guttman

• Publisher: John Wiley and Sons
• Year: 1964
• Tongue: English
• Weight: 319 KB
• Volume: 53
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600530919

✦ Synopsis

A study was made of the hydrolytic behavior of riboflavin in aqueous solutions buffered with a borate buffer system. It was observed that the vitamin decomposed slower in the presence of the b d e r e d rather than unbuffered solutions of equivalent hydroxide-ion concentration. Rate studies suggested that a reversible 1 : 1 association occurred between riboflavin and a component of the buffer to yield a product which underwent hydrolytic cleavage of the isoalloxazine ring at a slower rate than the parent compound. Similar studies with lumiflavin failed to demonstrate this effect and showed that the ribityl side-chain of riboflavin was involved in the association.