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Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation

✍ Scribed by Dixon, Ray ;Kennedy, Christina ;Kondorosi, Ad�m ;Krishnapillai, Viji ;Merrick, Mike

Publisher
Springer
Year
1977
Tongue
English
Weight
942 KB
Volume
157
Category
Article
ISSN
0026-8925

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✦ Synopsis

A series of mutants defective in nitrogen fixation (nif) were isolated in Klebsiella pneumoniae strain M5a1. The nif mutations were either located on plasmid pRD1 or on the K. pneumoniae chromosome. A total of 37 plasmid mutants and 28 chromosomal mutants were employed in complementation tests using the acetylene reduction technique. Most mutants could be assigned to one of seven nif cistrons: nifA, nifB, nifD, nifE, nifF, nifH, and nifK. Complementation analysis of two nif deletion mutants confirmed transductional evidence that these strains carry nifB-A-F deletions. One deletion mutant had, in contrast to previous transductional analysis, a functional nifK cistron and presumably is deleted for nifB-A-F-E. Examination of the biochemical phenotype of several mutants suggests that the nifA product has a regulatory function, and nifK, nifD and nifH are most probably the structural genes for nitrogenase.

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