Role of metal ions in negative regulation of nitrogen fixation by the nifL gene product from Klebsiella pneumoniae

Cloning of the glnA, ntrB and ntrC genes

Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster

✍ Espin, Guadalupe ;Alvarez-Morales, Ariel ;Cannon, Frank ;Dixon, Ray ;Merrick, Mi 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 696 KB

The glnA, ntrB and ntrC genes of Klebsiella pneumoniae have been cloned, on a 12 kb HindIII fragment, into the plasmid pACYC184. In a coupled in vitro transcription/translation system the resultant plasmid, pGE100, directed synthesis of five polypeptides (molecular weights 73, 53, 51, 39, 36 kd) fro