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Cloning of the entire region for nitrogen fixation from Klebsiella pneumoniae on a multicopy plasmid vehicle in Escherichia coli

✍ Scribed by Pühler, A. ;Burkardt, H. J. ;Klipp, W.


Publisher
Springer
Year
1979
Tongue
English
Weight
980 KB
Volume
176
Category
Article
ISSN
0026-8925

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The btuB gene of Escherichia coli codes for a protein (BtuB) located in the outer membrane. BtuB is the receptor for vitamin B12 (cyanocobalamin). We have cloned the btuB gene into pUC8 using transposon Tn5 as the marker to first isolate several parts of the relevant DNA fragment from the specialize