Competitor DNA template for quantification of hepatitis B virus by PCR
β Scribed by Aneta Bzorska; Urszula Lewandowska; Barbara Jozwiak; Janusz Greger
- Book ID
- 118565728
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 215 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0168-8278
No coin nor oath required. For personal study only.
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## Abstract A sensitive and reproducible realβtime PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an βinβhouseβ qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B pati
## Abstract The application of a new fluorogenic probeβbased PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between pr