๐”– Bobbio Scriptorium
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Competence in Bacillus subtilis Transformation System

โœ Scribed by YOUNG, FRANK E.


Book ID
109655667
Publisher
Nature Publishing Group
Year
1967
Tongue
English
Weight
410 KB
Volume
213
Category
Article
ISSN
0028-0836

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๐Ÿ“œ SIMILAR VOLUMES


Transformation in Bacillus subtilis
โœ Arwert, F. ;Venema, G. ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Springer ๐ŸŒ English โš– 787 KB
Plasmid transformation in Bacillus subti
โœ Bensi, G. ;Iglesias, A. ;Canosi, U. ;Trautner, T. A. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 859 KB

A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived

Plasmid transformation in Bacillus subti
โœ Iglesias, A. ;Bensi, G. ;Canosi, U. ;Trautner, T. A. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 573 KB

Various alterations (deletions, additions, inversions) were introduced into portions of pC194/B. subtilis or pC194/phi 105 hybrid plasmid molecules which are homologous to the DNA of recipients in transformation. These plasmids are stably maintained in transformations of recombination deficient cell

Plasmid transformation in Bacillus subti
โœ Iglesias, Antonio ;Ceglowski, Piotr ;Trautner, Thomas A. ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 666 KB

Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr

The initial attachment of transforming D
โœ Weppner, William A. ;Leach, Franklin R. ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 690 KB

The initial attachment of transforming DNA to competent Bacillus subtilis is temperature independent between 25 degrees and 45 degrees. However, below 15 degrees there is a significant reduction in the amount of DNA attached to compentent cells. The DNA that is attached at 4 degrees can lead to tran