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Comparison of three power supplies used for the single-cell gel assay

✍ Scribed by Maria Vrzoc; Michael Petras

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 308 KB
Volume: 28
Category: Article
ISSN: 0893-6692
DOI: 10.1002/(sici)1098-2280(1996)28:2<154::aid-em12>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

a n a d a

The alkaline singlecell gel (SCG) assay, which d e tail length to width ratios and profiles of these ratios termines DNA damage in mice treated with 100 mg/kg methyl methanesulfonate (MMS), was run by using three power supplies from BieRad (models 3000Xi, 200/2.0, and Power Pac 300). Comparisons of the results obtained from the use of these power supplies showed differences in mean DNA in indiGidual cells. Model 200/2.0 power supply appeared to enhance significantly the sensitivity of the assay. In purchasing power supplies, there is a need to evaluate their effect on the sensitivity of the test.

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