Comparative biomonitoring study of workers at a waste disposal site using cytogenetic tests and the comet (single-cell gel) assay
✍ Scribed by Andreas Hartmann; Helga Fender; Günter Speit
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 142 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
Workers exposed to environmental pollutants at a fragments and the percentage of aberrant cells (exwaste disposal site were studied for genotoxic ef-cluding gaps) was significantly increased. No signififects with cytogenetic tests and the comet (alkaline cant difference was found for the mean frequency of single-cell gel) assay. Analyses were performed on SCE. A statistically significant difference was also seen peripheral blood samples of 44 workers at a waste with the comet assay. The mean tail moment was disposal site (DM) and 47 subjects of a control higher in DM than in VE. However, no correlation was group (VE) matched for gender, age, and smoking found between cytogenetic data and the effects in the habits. Chromosomal aberrations were evaluated comet assay. The results of our study indicate that DNA in 1,000 lymphocytes per individual, sister chroma-effects in the comet assay represent an independent tid exchanges in 50 cells, and DNA migration (tail endpoint which might be useful for the biomonitormoment) was determined in 100 leukocytes. Struc-ing of genotoxic effects in addition to established tural chromosome aberrations were more frequent tests.