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Comparison of the requirements for ribonucleic acid synthesis with the requirements for deoxyribonucleic acid synthesis in animal tissues

✍ Scribed by J. J. Furth; Martin Rosenberg; Patricia L. Ho

Publisher: John Wiley and Sons
Year: 1967
Tongue: English
Weight: 638 KB
Volume: 69
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040690211

No coin nor oath required. For personal study only.

✦ Synopsis

Ribonucleic acid polymerase and deoxyribonucleic acid polymerase have been partially purified from bovine lymphosarcoma, lymph node, and thymus. An examination of the deoxyribonucleic acid requirements of the two enzymes indicates that ''native" deoxyribonucleic acid is the preferred template for ribonucleic acid synthesis; heat-denatured deoxyribonucleic acid is considerably less active. The primer requirements for deoxyribonucleic acid synthesis differ: "native" deoxyribonucleic acid is usually inactive, while denatured deoxyribonucleic acid is active. The two enzymes also differ in pH optima and in their requirements for metal cofactors.

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