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Comparison of protocols for pulsed-field gel electrophoresis of Clostridia

✍ Scribed by Andreas Stolle; Brigitte Sperner; Barbara Schalch; Hartmut Eisgruber

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
103 KB
Volume
22
Category
Article
ISSN
0173-0835

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The velocity and orientation of T4 and X DNA have been measured for the first 20 s during pulsed-field gel electrophoresis in order to clarify the DNA motions that occur. For a square pulse with field strength E = 10 V/cm, the velocity of X DNA increases gradually to 10.5 pm/s in 1.0 s, declines to