Progress in developing improved programs for pulsed field agarose gel electrophoresis of DNA
โ Scribed by Malik F. Arshad; Frederick J. Dunn; Raul Vega; Jonathan W. Valvano; Dr. Philip Serwer
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 588 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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It has been reported that secondary pulsed field gel (SPFG) electrophoresis can dramatically increase the speed of separation of large DNA molecules without a decrease in resolution (Zhang, T. Y., Smith, C. L., and Cantor, C. R. (1991) Nucleic Acids Res. 19, \(1291-\) 1296). However, our attempts to
Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa