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Comparison of micro-LC and capillary zone electrophoresis for the analysis of thiols

✍ Scribed by Ling, B. Lin ;Baeyens, W. R. G. ;Dewaele, C.

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
458 KB
Volume
14
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Micro‐LC and capillary zone electrophoresis (CZE) are two of the most rapidly developing, miniaturized separation techniques in analytical science. In the present paper, both techniques were comparatively evaluated for the determination of several important thiols derivatized with the fluorogenic reagent SBD‐F. Different parameters, such as selectivity, sensitivity, analysis time and efficiency, influence of column length, CZE buffer concentration, and voltage were thus analyzed and discussed. Both techniques are recommended for the analysis of thiols in biological samples.

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