✦ LIBER ✦

Analysis of thiol-containing compounds in biological samples by capillary zone electrophoresis

✍ Scribed by Nuran Ercal; Kang Le; Piyanee Treeratphan; Richard Matthews

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 322 KB
Volume: 10
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199601)10:1<15::aid-bmc544>3.0.co;2-5

No coin nor oath required. For personal study only.

✦ Synopsis

A new method for the determination of reduced and oxidized glutathione by using capillary zone electrophoresis was developed and compared with the N-( 1-pyreny1)maleimide high-performance liquid chromatography method. The tissue samples were obtained from C57BL16 mice and homogenized in deionized water. The detection wavelength was set at 214 nm. A 50-cm effective length X 75 pm i.d. uncoated fused silica capillary with a 10 mM phosphate buffer at pH 7.5 was used for the experiment. Samples were introduced by pressure injection for 5 s. The voltage for separation was at 20 kV. Between runs, the capillary was regularly washed with 0.1 N NaOH and deionized water, followed by reconditioning with running buffer. The present method was shown to be reproducible and is convenient in that a derivatization step is not required. The method has been used to measure glutathione levels in biological samples and the results show good agreement with those obtained by high-performance liquid chromotography.

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