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Comparison of methods for aflatoxin analysis by high-pressure liquid chromatography

โœ Scribed by Larry M. Seitz

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
569 KB
Volume
104
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

Reversed-phase columns packed with octadecyl and phenyl reversed phases did not provide adequate separation of aflatoxins. A pellicular adsorption column provided partial separation, i.e. B1 and Bz from GI and G1, but not BI from I& nor G1 from Gz. A microparticulate adsorption (Micro-A) column completely separated aflatoxins B,, B2, GI, and G1. Detection was more selective at 350 nm (or 365 nm) than at 254 nm. A Fluoro Monitor Model 1209 detector (Laboratory Data Control

Corp.) was more sensitive for aflatoxins G1 and Gz than for B1 and Bz. Aflatoxin B, at the 30-ppb level in yellow corn was detected with the Micro-A column and the 350-nm photometer. The limit of detection was estimated at about 10 ppb"".

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