Determination of nitrate and nitrite by high-pressure liquid chromatography: Comparison with other methods for nitrate determination

✍ Scribed by J.R. Thayer; R.C. Huffaker

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 788 KB
• Volume: 102
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90325-5

✦ Synopsis

A fast, simple, and very sensitive method for the simultaneous determination of nitrate and nitrite was developed by coupling the high ultraviolet absorbance of these compounds with their separation from one another and from components of media and cell extracts by highpressure anion-exchange liquid chromatography. This method permits rapid determination of as little as 50 pmol of nitrate and 200 pmol of nitrite in volumes from 5 to 500 ~1. The effects of alterations in the flow rate, pH, and ionic strength ofthe mobile phase, changes in the injection volume, and use of guard columns were investigated, and use of the method for determining some organic compounds was examined. The method was compared with other methods for nitrate determination.