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Comparison of conformation sensitive gel electrophoresis with denaturing gradient gel electrophoresis for detection of single-base mutations in PCR products

✍ Scribed by J. Körkkö; T. Pihlajamaa; D.J. Prockop; L. Ala-Kokko

Book ID: 117640422
Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 90 KB
Volume: 15
Category: Article
ISSN: 0945-053X
DOI: 10.1016/s0945-053x(96)90057-8

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More than 500 mutations have been identified in the CFTR gene, making it an excellent system for testing mutation scanning techniques. To assess the sensitivity of denaturing gradient gel electrophoresis (DGGE), we collected a representative group of 202 CFTR mutations. All mutations analyzed were d