A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations
β Scribed by John S. Beck; Anne E. Kwitek; Phillip H. Cogen; Andrew K. Metzger; Geoffrey M. Duyk; Val C. Sheffield
- Book ID
- 104674046
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 651 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0340-6717
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## Abstract Structural alterations of the __p53__ gene were investigated in chemically induced rat hepatocellular carcinomas (HCCs), hyperplastic hepatic nodules (HPNs), and cell lines derived from rat neoplastic and normal liver cells. The mutations were detected by GCβclamped denaturing gradient
Screening for mutations in disease-related genes (e.g., protooncogenes) is an important challenge, since even point mutations can lead to constitutive activation of the corresponding gene product. This can result in cell transformation, as in the case of the ras genes (1). ## FIG. 1. Mapping of t