✦ LIBER ✦
Mutation Detection in Mosaic Situations: RNA Mismatch Assay and Denaturing Gradient Gel Electrophoresis Are More Sensitive Than Conventional Cycle Sequencing
✍ Scribed by Sandra Dunger; Susanne Neumann; Roland Zell; Eckhard Birch-Hirschfeld; Axel Stelzner; Ralf Paschke; Raimund W. Kinne; Stefan Sickinger
- Book ID
- 102559411
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 189 KB
- Volume
- 294
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Screening for mutations in disease-related genes (e.g., protooncogenes) is an important challenge, since even point mutations can lead to constitutive activation of the corresponding gene product. This can result in cell transformation, as in the case of the ras genes (1).
FIG. 1.
Mapping of the 3Ј (A) and 5Ј (B) ends of yeast mitochondrial 16S rRNA. Lanes 1, mapping product; lanes 2, negative control (mapping without RNA); lanes 3, free probe. 1 g of mitochondrial RNA was used for hybridization.