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DDGGE, a simplified denaturing gradient gel electrophoresis method for the detection of sequence variations in PCR fragments

✍ Scribed by Susanne Logemann; Mike S.M. Jetten


Book ID
110269511
Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
457 KB
Volume
12
Category
Article
ISSN
0951-208X

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## Communicated by Jean-Claude Kuplan We developed a parallel denaturing gradient gel electrophoresis (DGGE) protocol to maximize the detection of nucleotide variants in the D N A sequence coding for the mature insulin receptor and in splice site junctions. The melting behaviours of exons 2 to 22