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Evaluation of denaturing gradient gel electrophoresis in the detection of 16S rDNA sequence variation in rhizobia and methanotrophs

✍ Scribed by Tatiana Vallaeys; Edward Topp; Gerard Muyzer; Valérie Macheret; Gisèle Laguerre; Annabel Rigaud; Guy Soulas


Book ID
104308102
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
865 KB
Volume
24
Category
Article
ISSN
0168-6496

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✍ Christèle Desbois; Jocelyne Magré; Véronique Blanquet; Jacqueline Capeau; Michel 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 842 KB

## Communicated by Jean-Claude Kuplan We developed a parallel denaturing gradient gel electrophoresis (DGGE) protocol to maximize the detection of nucleotide variants in the D N A sequence coding for the mature insulin receptor and in splice site junctions. The melting behaviours of exons 2 to 22