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Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase

✍ Scribed by Fui-Ling Wong; Azila Abdul-Aziz

Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
136 KB
Volume
83
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chemically‐linked PVA, freeze‐thawed PVA cryogel, tetramethoxysilane (TMOS) sol‐gel‐PVA hybrid material, and alumina sol‐gel‐PVA hybrid material.

RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross‐linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K~m~^app^.

CONCLUSION: Freeze‐thawed PVA‐GOD membranes and TMOS‐PVA, which showed satisfactory sensitivity and adequate value of K~m~^app^, were quite promising as support materials for immobilizing GOD. Copyright © 2007 Society of Chemical Industry

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