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Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod

✍ Scribed by Michel Monsigny; Claire Petit; Annie-Claude Roche

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
451 KB
Volume
175
Category
Article
ISSN
0003-2697

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✦ Synopsis

A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.

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