✦ LIBER ✦

Colorimetric micromethods for glutaraldehyde determination by means of phenol and sulfuric acid or phenol and perchloric acid

✍ Scribed by Janusz Boratyński; Tomasz Żal

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 391 KB
Volume: 184
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90677-2

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✦ Synopsis

Aqueous micromolar solutions of glutaraldehyde produce a yellow color when treated with 20% phenol in ethanol followed by concentrated sulfuric acid or with phenol in 70% perchloric acid. The absorbance at 482 or 479 nm, respectively, is linearly related to the glutaraldehyde concentration. Of the two methods developed, the sulfuric acid-phenol assay gives a higher sensitivity and the perchloric acid-phenol assay allows the determination of glutaraldehyde in the presence of sugars and proteins.

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