Colorimetric assay of 2,2-bis(4-chlorophenyl)acetic acid (DDA) in urine

✍ Scribed by S.S.A. Zaidi; B.D. Banerjee; M. Ramachandran; Q.Z. Hussain

• Book ID: 102628428
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 164 KB
• Volume: 136
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90322-1

✦ Synopsis

2,2-Bis(4-chlorophenyl) acetic acid (DDA) was extracted from acidified urine at pH 2 by benzene. It was complexed by partitioning with crystal violet in acetic acid to yield a blue color which was quantitated at 6 10 nm. Its identity was conlirrned by paper chromatographic separation. The DDA excretion in nine subjects ranged from 0.024 to 0.130 &ml with a mean of 0.065 while that determined by gas chromatography ranged from 0.038 to 0.120 with a mean of 0.069.