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Development of an enzyme-linked immunosorbent assay for the quantification of DDA (2,2-bis(p-chlorophenyl) acetic Acid) in urine

โœ Scribed by B. D. Banerjee


Book ID
112576143
Publisher
Springer
Year
1987
Tongue
English
Weight
344 KB
Volume
38
Category
Article
ISSN
0007-4861

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Colorimetric assay of 2,2-bis(4-chloroph
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2,2-Bis(4-chlorophenyl) acetic acid (DDA) was extracted from acidified urine at pH 2 by benzene. It was complexed by partitioning with crystal violet in acetic acid to yield a blue color which was quantitated at 6 10 nm. Its identity was conlirrned by paper chromatographic separation. The DDA excret