Colorimetric Assay for Dipicolinic Acid in Bacterial Spores
โ Scribed by JANSSEN, F. W.; LUND, A. J.; ANDERSON, L. E.
- Book ID
- 121164003
- Publisher
- American Association for the Advancement of Science
- Year
- 1958
- Tongue
- English
- Weight
- 492 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0036-8075
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๐ SIMILAR VOLUMES
Dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mM HCl for 10 min at 100 degrees C. The suspension was diluted with 5 mM Ca2+, 100 mM Tris, pH 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285
The occurrence of substantial amounts of dipicolinic acid (DPA) in bacterial spores, the idea that DPA is related in some yet-to-be-explained way to the extraordinary heat stability of these cells, and the lack of knowledge of any function for this biologically unusual compound make a rapid accurate
Curie-point pyrolysis/triple quadrupole mass spectrometry and micro-tube furnace pyrolysis/quadrupole ion trap mass spectrometry have been used to detect dipicolinic acid (DPA) in sporulated whole bacteria. DPA in whole cells of sporulated Bacillus anthracis reacted in situ during pyrolysis with tet