๐”– Bobbio Scriptorium
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Ultraviolet absorption by dipicolinic acid in model systems and bacterial spores

โœ Scribed by V.H. Holsinger; L.C. Blankenship; M.J. Pallansch

Book ID
118845769
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
390 KB
Volume
119
Category
Article
ISSN
0003-9861

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The occurrence of substantial amounts of dipicolinic acid (DPA) in bacterial spores, the idea that DPA is related in some yet-to-be-explained way to the extraordinary heat stability of these cells, and the lack of knowledge of any function for this biologically unusual compound make a rapid accurate

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Dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mM HCl for 10 min at 100 degrees C. The suspension was diluted with 5 mM Ca2+, 100 mM Tris, pH 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285

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