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Coexpression of tumor-associated α2-macroglobulin and growth factors in human melanoma cell lines

✍ Scribed by Alena Lizonová; Jozef Bízik; Marta Grófóva; Antti Vaheri

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
854 KB
Volume
43
Category
Article
ISSN
0730-2312

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✦ Synopsis

a,-Macroglobulin ( a 2 M ) is known as an inhibitor of various proteinases and to bind several of the growth factors. W e previously demonstrated that clonal variation exists in the production of a,M in a human melanoma and that this variation may be associated with growth stimulation. We have now analyzed six human melanoma cell lines for the simultaneous expression of TGF-a, TGF-0, PDGF-A chain, PDGF-B chain, and tumor-associated a,M. In Northern blot analysis TGF-a was detected in four of the cell lines, TGF-P in all, PDGF-A chain in three, and PDGF-B chain in none of the cell lines. a 2 M , detected by immunoblotting, varied significantly between the different melanoma cell lines and only one cell line was found to be negative. Evaluation of growth-promoting activity in conditioned media suggested that a,-macroglobulin, secreted by these tumor cell lines, is a significant modulator of melanoma cell growth.

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