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Coenzymatic Activity of Pyridoxal 5′-sulfate and Related Analogues of Pyridoxal 5′-phosphate

✍ Scribed by E. Groman, Y. Z. Huang, T. Watanabe and Esmond E. Snell


Book ID
123650929
Publisher
National Academy of Sciences
Year
1972
Tongue
English
Weight
837 KB
Volume
69
Category
Article
ISSN
0027-8424

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Fluorometric assay of pyridoxal and pyri
✍ M.S. Chauhan; K. Dakshinamurti 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 517 KB

A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly