We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5-phosphoribosyl-4carboxamide 5-aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupted open reading frame of 1755 nucleotides corresponding to 585 amino acid residues. The de
Cloning theSchizosaccharomyces pombelys2+gene and construction of new molecular genetic tools
β Scribed by Richard L. Hoffman; Charles S. Hoffman
- Publisher
- Springer-Verlag
- Year
- 2006
- Tongue
- English
- Weight
- 419 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The genetic organisation of the ruv gene, a component of the SOS system for DNA repair and recombination in Eseheriehia toll, was investigated. New point mutations as well as insertions and deletions were generated using transposon TnlO inserted in eda as a linked marker for site specific mutagenesi
The activity of the nuclear gene PET494 is required to allow expression of the yeast mitochondrial gene oxi2. To aid the study of the mechanism of action of PET494 we have isolated this gene from yeast DNA. A clone bank of yeast DNA fragments in a yeast-E. coli shuttle vector was screened by transfo
The 6.5 kb HindIII DNA fragment of the Lactococcus lactis subsp. cremoris H2 plasmid pDI21 was cloned into Escherichia coli POP13 with lambda NM1149, and also directly into Lactococcus lactis subsp. lactis 4125 using a newly-constructed broad host-range vector pFX1. Proteinase was expressed in both