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Cloning and manipulation of theSchizosaccharomyces pombe his7+gene as a new selectable marker for molecular genetic studies

✍ Scribed by Ethel Apolinario; Mary Nocero; Mei Jin; Charles S. Hoffman

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
608 KB
Volume
24
Category
Article
ISSN
0172-8083

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Genetic engineering of Schizosaccharomyc
Genetic engineering of Schizosaccharomyces pombe: A system for gene disruption and replacement using the ura4 gene as a selectable marker
✍ Grimm, Christian ;Kohli, JΓΌrg ;Murray, Johanne ;Maundrell, Kinsey πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 702 KB

A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,