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Cloning, sequencing and complementation analysis of the recA gene from Prevotella ruminicola

✍ Scribed by Rustern I. Aminov; Takafumi Nagamine; Koretsugu Ogata; Mutsumi Sugiura; Kiyoshi Tajima; Yoshimi Benno

Book ID
109321362
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
560 KB
Volume
144
Category
Article
ISSN
0378-1097

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Molecular cloning and nucleotide sequenc
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A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV ir