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Sequencing and complementation analysis of the nifUSV genes from Azospirillum brasilense

โœ Scribed by Jeverson Frazzon; Irene Silveira Schrank


Book ID
109322109
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
505 KB
Volume
159
Category
Article
ISSN
0378-1097

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A cluster of four Azospirillum brasilense histidine biosynthetic genes, hisA, hisB, hisF and hisH, was identified on a 4.5 kb DNA fragment and its organization studied by complementation analysis of Escherichia coli mutations and nucleotide sequence. The nucleotide sequence of a 1.3 kb fragment that

Nucleotide sequence of the gene encoding
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The DNA sequence was determined for the Azospirillum brasilense nifH gene and part of the nifD gene. The nifH gene is 885 bp long and encodes 293 amino acid residues. The region upstream of the nifH open reading frame contains a putative promoter whose sequence shows perfect homology with promoters