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Cloning of theRhizomucor miehei3-hydroxy-3-methylglutaryl-coenzyme A reductase gene and its heterologous expression inMucor circinelloides

✍ Scribed by Gyöngyi Lukács; Tamás Papp; Ferenc Somogyvári; Árpád Csernetics; Ildikó Nyilasi; Csaba Vágvölgyi


Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
400 KB
Volume
95
Category
Article
ISSN
0003-6072

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We have used a cDNA clone for Chinese hamster 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase to isolate a genomic recombinant for human HMG-CoA reductase. The identity of the gene was confirmed by partial sequence analysis. Several unique fragments that will be useful for restriction frag