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Cloning of theAspergillus oryzae5-aminolevulinate synthase gene and its use as a selectable marker

โœ Scribed by Susan L. Elrod; Aubrey Jones; Randy M. Berka; Joel R. Cherry


Book ID
106006004
Publisher
Springer-Verlag
Year
2000
Tongue
English
Weight
228 KB
Volume
38
Category
Article
ISSN
0172-8083

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โœ Bai, Xiyuan; Larsen, Michael; Meinhardt, Friedhelm ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 264 KB ๐Ÿ‘ 2 views

A pair of degenerate primers was used for amplification and cloning of an internal fragment of the K. lactis URA5 gene. Primers were designed on the basis of highly conserved motifs within protein sequences predicted for URA5 genes from several microorganisms. Using the amplified fragment as a probe