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Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker

โœ Scribed by Der Thor; See Xiong; Claire C. Orazem; An-Chun Kwan; James M. Cregg; Joan Lin-Cereghino; Geoff P. Lin-Cereghino


Book ID
108131386
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
346 KB
Volume
5
Category
Article
ISSN
1567-1356

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Cloning and characterization of the Pich
โœ Ohi, Hideyuki; Ohtani, Wataru; Okazaki, Noriko; Furuhata, Naoto; Ohmura, Takao ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 893 KB

We purified a 58 kDa serine protease from culture-supernatant of Pichia pastoris and found that the NH,-terminal amino acid sequence of this protease is closely homologous to that of mature protein of Saccharomyces cerevisiae carboxypeptidase Y (CPY), which is encoded by the PRCl gene. Using the S.