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Cloning of the High Alkaline Endoglucanase Gene from Bacillus pumilus AC-4 and Expressed in E.coli

โœ Scribed by Lin, Yuan; Li, Xin Zhou; Gao, Ji Wei; Xu, Jing; Du, Chao

Book ID
120449312
Publisher
Trans Tech Publications Inc.
Year
2012
Tongue
English
Weight
370 KB
Volume
249-250
Category
Article
ISSN
1660-9336

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Molecular cloning of the genes for xylan
Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli
โœ Panbangred, Watanalai ;Kondo, Tetsuya ;Negoro, Seiji ;Shinmyo, Atsuhiko ;Okada, ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 767 KB

The 7.7 Mdal PstI fragment of Bacillus pumilus IPO containing genes for xylan degradation, xylanase, and fl-xylosidase was inserted at the Pstl site of pBR322 and cloned in E. coli C600. The hybrid plasmid thus formed was named pOXN29. The amount of xylanase and fl-xylosidase expressed in E. coli ha