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Cloning and expression of alkaline protease genes from two salt-tolerant alkaliphilic actinomycetes in E. coli

โœ Scribed by S.D. Gohel; S.P. Singh


Book ID
116542676
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
941 KB
Volume
50
Category
Article
ISSN
0141-8130

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A gene, aprP, encoding an extracellular alkaline serine protease from a newly isolated Pseudomonas sp. KFCC 10818 was cloned and characterized. Nucleotide sequence analysis revealed an open reading frame of 1,266 nucleotides which could encode a polypeptide comprised of 422 amino acids. The C-termin