Cloning of the ethidium efflux gene from Escherichia coli
โ Scribed by A.S. Purewal; I.G. Jones; M. Midgley
- Book ID
- 109316460
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 213 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Specific xylose utilization mutants of Escherichia coli were isolated that had altered xylose isomerase ( xylA ), xylulokinase ( xylB ), and regulatory ( xylR ) or transport ( xylT ) activities. We screened the Clarke and Carbon E. coli gene bank and one clone, pLC10 -15, was found to complement the
A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene
Mutations in the fnr gene of Escherichia coli have pleiotrophic effects leading to deficiencies in the reduction of fumarate and nitrate, hydrogen production and the ability to grow anaerobically with fumarate or nitrate as terminal electron acceptors. Transducing phages (lambda fnr) carrying the wi