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The cloning of the rorB gene of Escherichia coli

✍ Scribed by Debenham, Paul G. ;Webb, Michael B. T. ;Law, John

Publisher
Springer
Year
1988
Tongue
English
Weight
457 KB
Volume
215
Category
Article
ISSN
0026-8925

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✦ Synopsis

A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.

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