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Cloning and characterization of the xyl genes from Escherichia coli

✍ Scribed by Rosenfeld, Stuart A. ;Stevis, Panayiotis E. ;Ho, Nancy W. Y.

Publisher
Springer
Year
1984
Tongue
English
Weight
772 KB
Volume
194
Category
Article
ISSN
0026-8925

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✦ Synopsis

Specific xylose utilization mutants of Escherichia coli were isolated that had altered xylose isomerase ( xylA ), xylulokinase ( xylB ), and regulatory ( xylR ) or transport ( xylT ) activities. We screened the Clarke and Carbon E. coli gene bank and one clone, pLC10 -15, was found to complement the xyl mutants we had characterized. Subcloning and DNA restriction mapping allowed us to locate the xylA and xylB genes on a 1.6 kbp Bg/II fragment and a 2.6 kbp HindIII-Sa/I fragment, respectively. The identification and mapping of xyl gene promoters suggest that the xylA and xylB genes are organized as an operon having a single xylose inducible promoter preceding the xylA gene.

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