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Cloning of the histidine transport genes from salmonella typhimurium and characterization of an analogous transport system in escherichia coli

✍ Scribed by Ardeshir, Feroza ;Ames, Giovanna Ferro-Luzzi

Publisher
Wiley (John Wiley & Sons)
Year
1980
Tongue
English
Weight
993 KB
Volume
13
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

The genes for the well‐characterized high‐affinity histidine transport system of S typhimurium have been cloned in λgt4. Genetic and physiological analyses of the analogous transport system of E coli were undertaken in order that available λ vectors, recombinant DNA techniques, and a genetic selection for transport function might be used to isolate the Salmonella genes. The presence of the transport genes on a 12.4 Kb cloned DNA fragment has been confirmed (1) genetically, by complementation studies; (2) physiologically, by the rates of histidine uptake by bacteria containing this DNA; and (3) by demonstrating that the cloned DNA codes for the previously identified transport proteins J and P. The isolated fragment carries the entire transport operon, the argT gene and the ubiX locus, but neither the purF gene nor the ack/pta loci.

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Reconstitution of binding protein dependent ribose transport in spheroplasts derived from a binding protein negative escherichia coli K12 mutant and from salmonella typhimurium
✍ Robb, Frank T. ;Furlong, Clement E. 📂 Article 📅 1980 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 437 KB

## Abstract Highly purified ribose‐binding protein from Escherichia coli has been used to reconstitute a binding‐protein‐dependent ribose transport in spheroplasts derived from a binding‐protein‐deficient mutant of E coli K 12, and in spheroplasts derived from Salmonella typhimurium. The cross‐spec