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Cloning of the carbohydrate-binding portion of the toxin A gene ofClostridium difficile

✍ Scribed by Stuart B. Price; Carol J. Phelps; Tracy D. Wilkins; John L. Johnson

Publisher
Springer
Year
1987
Tongue
English
Weight
961 KB
Volume
16
Category
Article
ISSN
0343-8651

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Cloning ofClostridium difficiletoxin B g
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✍ Christoph Eichel-Streiber; Rita Laufenberg-Feldmann; Sabine Sartingen; JΓΆrg Schu πŸ“‚ Article πŸ“… 1990 πŸ› Springer-Verlag 🌐 English βš– 671 KB

High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HinclI fragment of chrom