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Cloning and expression of the toxin B gene ofClostridium difficile

✍ Scribed by John L. Johnson; Carol Phelps; Lisa Barroso; Mary D. Roberts; David M. Lyerly; Tracy D. Wilkins

Publisher
Springer
Year
1990
Tongue
English
Weight
856 KB
Volume
20
Category
Article
ISSN
0343-8651

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Cloning ofClostridium difficiletoxin B g
Cloning ofClostridium difficiletoxin B gene and demonstration of high N-terminal homology between toxin A and B
✍ Christoph Eichel-Streiber; Rita Laufenberg-Feldmann; Sabine Sartingen; JΓΆrg Schu πŸ“‚ Article πŸ“… 1990 πŸ› Springer-Verlag 🌐 English βš– 671 KB

High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HinclI fragment of chrom