Cloning ofClostridium difficiletoxin B gene and demonstration of high N-terminal homology between toxin A and B
✍ Scribed by Christoph Eichel-Streiber; Rita Laufenberg-Feldmann; Sabine Sartingen; Jörg Schulze; Markus Sauerborn
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 671 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0300-8584
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✦ Synopsis
High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HinclI fragment of chromosomal DNA overlaps both groups of clones. A partial restriction map of the total toxB gene is presented. The gene is positioned upstream of utxA and toxA. toxB has a size of 6.9 kb, corresponding to a 250-kDa polypeptide. A partial sequence of the 5' end of toxB was determined. The sequence contains 398 bp upstream of toxB with a putative Shine-Dalgarno box (AGGAGA) and 609 bp of the toxB open reading frame. The N-terminal 203 amino acids of ToxB were compared with the N-terminal amino acids of the enterotoxin A (ToxA). A homology of 64% of the residues was detected, which proves the relatedness of ToxA and ToxB of C. difficile.