Cloning of Ruminococcus albus endo-β-1,4-glucanase and xylanase genes
✍ Scribed by M.P.M. Romaniec; K. Davidson; B.A. White; G.P. Hazlewood
- Book ID
- 115271491
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 263 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0266-8254
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An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular
New soluble chromogenic substrates were prepared for specific and rapid assays of endo-l,4-/3-xylanases and endo-1 ,4+glucanases. A soluble beechwood 4-0-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulos was coupled to Ostazin brilliant red H-3B. The assays