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Modification of an Endo-1,4-β-Glucanase from Ruminococcus albus by Gene Truncation

✍ Scribed by KUNIO OHMIYA; HIROYUKI DEGUCHI; TSUTOMU KAJINO; SHOICHI SHIMIZU


Book ID
119864292
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
406 KB
Volume
613
Category
Article
ISSN
0890-6564

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Purification and properties of endo-(1→4
✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular