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Cloning of Lentinus edodes mitochondrial dna fragment capable of autonomous replication in Saccharomyces cerevisiae

✍ Scribed by Yuichi Katayose; Kazuo Shishido; Masatake Ohmasa

Book ID
117057192
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
543 KB
Volume
138
Category
Article
ISSN
0006-291X

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A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24AEcoRl and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from t