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Autonomously replicating fragments of mitochondrial DNA from Penicillium urticae in Saccharomyces cerevisiae

✍ Scribed by Hiroki Yamamoto; Youko Yoshida; Yasunori Kitamura; Junichi Sekiguchi

Book ID
113396633
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
354 KB
Volume
71
Category
Article
ISSN
0922-338X

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A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24AEcoRl and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from t