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DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae

✍ Scribed by RNDr. František Jurský; Eva Hostinová

Book ID: 102393943
Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 234 KB
Volume: 30
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620300419

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✦ Synopsis

A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24AEcoRl and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saecharomycopsisfibuligera with ARS activity in Saccharomyces cerevisiae.

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